ASTM E1881-2006 用次级离子质谱法(SIMS)进行细胞培养分析的标准指南
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【英文标准名称】:StandardGuideforCellCultureAnalysiswithSIMS
【原文标准名称】:用次级离子质谱法(SIMS)进行细胞培养分析的标准指南
【标准号】:ASTME1881-2006
【标准状态】:现行
【国别】:
【发布日期】:2006
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E42.06
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:微生物学;规范(验收);频谱测定法
【英文主题词】:SIMS
【摘要】:ThepresenceofcellgrowthmediumcomplicatesadirectanalysisofcellswithSIMS.Attemptstowashoutthenutrientmediumresultsintheexposureofcellstounphysiologicalreagentsthatmayalsoaltertheirchemicalcomposition.Thisobstacleisovercomebyusingasandwichfreeze-fracturemethod(1).ThiscryogenicmethodhasprovidedauniquewayofsamplingindividualcellsintheirnativestateforSIMSanalysis.TheproceduredescribedherehasbeensuccessfullyusedforimagingNa+andK+iontransport(3),calciumalterationsinstimulatedcells(4,5),andlocalizationoftherapeuticdrugsandisotopicallylabeledmoleculesinsinglecells(6).Thefrozenfreeze-driedcellspreparedaccordingtothismethodhavebeencheckedforSIMSmatrixeffects(7).Ionimagequantificationhasalsobeenachievedinthissampletype(8).Theproceduredescribedhereisamenabletoawidevarietyofcellculturesandprovidesawayforstudyingtheresponseofindividualcellsforchemicalalterationsinthestateofhealthanddiseaseandlocalizationofisotopically-labeledmoleculesandtheraputicdrugsincellculturemodels.1.1ThisguideprovidestheSecondaryIonMassSpectrometry(SIMS)analystwithacryogenicmethodforanalyzingindividualtissueculturecellsgrowinginvitro.Thisguideissuitableforfrozen-hydratedandfrozen-freeze-driedsampletypes.Includedareproceduresforcorrelatingoptical,laserscanningconfocalandsecondaryelectronmicroscopiestocomplementSIMSanalysis.1.2Thisguideisnotsuitableforcellculturesthatdonotattachtothesubstrate.1.3Thisguideisnotsuitableforanyplasticembeddedcellculturespecimens.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C04;A40
【国际标准分类号】:07_100_10
【页数】:3P.;A4
【正文语种】:
【原文标准名称】:用次级离子质谱法(SIMS)进行细胞培养分析的标准指南
【标准号】:ASTME1881-2006
【标准状态】:现行
【国别】:
【发布日期】:2006
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E42.06
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:微生物学;规范(验收);频谱测定法
【英文主题词】:SIMS
【摘要】:ThepresenceofcellgrowthmediumcomplicatesadirectanalysisofcellswithSIMS.Attemptstowashoutthenutrientmediumresultsintheexposureofcellstounphysiologicalreagentsthatmayalsoaltertheirchemicalcomposition.Thisobstacleisovercomebyusingasandwichfreeze-fracturemethod(1).ThiscryogenicmethodhasprovidedauniquewayofsamplingindividualcellsintheirnativestateforSIMSanalysis.TheproceduredescribedherehasbeensuccessfullyusedforimagingNa+andK+iontransport(3),calciumalterationsinstimulatedcells(4,5),andlocalizationoftherapeuticdrugsandisotopicallylabeledmoleculesinsinglecells(6).Thefrozenfreeze-driedcellspreparedaccordingtothismethodhavebeencheckedforSIMSmatrixeffects(7).Ionimagequantificationhasalsobeenachievedinthissampletype(8).Theproceduredescribedhereisamenabletoawidevarietyofcellculturesandprovidesawayforstudyingtheresponseofindividualcellsforchemicalalterationsinthestateofhealthanddiseaseandlocalizationofisotopically-labeledmoleculesandtheraputicdrugsincellculturemodels.1.1ThisguideprovidestheSecondaryIonMassSpectrometry(SIMS)analystwithacryogenicmethodforanalyzingindividualtissueculturecellsgrowinginvitro.Thisguideissuitableforfrozen-hydratedandfrozen-freeze-driedsampletypes.Includedareproceduresforcorrelatingoptical,laserscanningconfocalandsecondaryelectronmicroscopiestocomplementSIMSanalysis.1.2Thisguideisnotsuitableforcellculturesthatdonotattachtothesubstrate.1.3Thisguideisnotsuitableforanyplasticembeddedcellculturespecimens.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C04;A40
【国际标准分类号】:07_100_10
【页数】:3P.;A4
【正文语种】:
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